Background: The fleshy root of Panax ginseng C.A. Meyer (ginseng) is one of the most well-known and valued herbs in traditional Chinese medicine. Ginsenosides are considered mainly responsible for the pharmacological activities of ginseng. The purpose of this study was to investigate the effects of ginsenoside-Rb 1 (G-Rb 1 ) on swimming exercise-induced oxidative stress in male mice. Materials and Methods: A total of 48 animals were randomly divided into four groups, with twelve mice in each group. The first, second and third groups were designed as G-Rb 1 treatment groups, got 25, 50 and 100 mg/kg bodyweight of G-Rb 1 , respectively. The fourth group was designed as the control group, got physiologic saline. The mice were intragastrically administered once daily for 4 weeks. The weight-loaded forced swimming test was conducted on the final day of experimentation. Then the exhaustive swimming time, blood lactate, serum creatine kinase (CK), malondialdehyde (MDA) and antioxidant enzymes in liver of mice were measured. Results: The results showed that G-Rb 1 could prolong the exhaustive swimming time and improve exercise endurance capacity of mice, as well as accelerate the clearance of blood lactate and decrease serum CK activities. Meanwhile, G-Rb 1 could decrease MDA contents and increase superoxide dismutase, catalase, glutathione peroxidase activities in liver of mice. Conclusions: The study suggested that G-Rb 1 possessed protective effects on swimming exercise-induced oxidative stress in mice.
Calphad: Computer Coupling of Phase Diagrams and Thermochemistry,2001年25(2):305-317 ISSN：0364-5916
[Zhang, YL] Cent S Univ, State Key Lab Powder Met, Changsha 410083, Hunan, Peoples R China.;Cent S Univ, PD Ctr Mat Sci & Engn, Changsha 410083, Peoples R China.
[Zhang, YL] Cent S Univ, State Key Lab Powder Met, Changsha 410083, Hunan, Peoples R China.
Annual Meeting of the Minerals-Metals-and-Materials-Society (TMS)
FEB 12-15, 2001
NEW ORLEANS, LA
Cent S Univ, State Key Lab Powder Met, Changsha 410083, Hunan, Peoples R China.^Cent S Univ, PD Ctr Mat Sci & Engn, Changsha 410083, Peoples R China.
The thermodynamic properties of the Nb-Ti system have been evaluated by using a regular solution model to describe the Gibbs energies of various phases including both equilibrium and metastable phases. A set of thermodynamic parameters more consistent with most of the selected experimental data than previous assessments has been obtained. Stable and metastable phase equilibria, T<inf>0</inf> loci, metastable chemical spinodal curve of βphase and thermodynamic properties are calculated with the optimized parameters. With the thermodynamic parameters obtained in this work, authors discuss the possible occurrence range of ωphase.
[Lin, JinYang] Cent S Univ, Dept Phys Educ, Changsha, Hunan, Peoples R China.;[Zhang, ZhuoYing] Hunan Normal Univ, Dept Phys Educ, Changsha, Hunan, Peoples R China.
International Conference on Chemical, Material and Food Engineering (CMFE)
JUL 25-26, 2015
Kunming, PEOPLES R CHINA
[Lin, JinYang] Cent S Univ, Dept Phys Educ, Changsha, Hunan, Peoples R China.^[Zhang, ZhuoYing] Hunan Normal Univ, Dept Phys Educ, Changsha, Hunan, Peoples R China.
AER-Advances in Engineering Research
flavonoids from Eucommia ulmoides;reduced glutathione;oxidized giutathione;superoxide disnuttase;catalase;malondiaklehyde;8-hydroxy-2 '-deoxyguanosine
Objective: this study examined the effects of flavonoids from Eucommia ulmoides (FEU) on antioxidant systems, lipid peroxidation and oxidative deoxyribonucleic acid (DNA) damage after exhaustive exercise. Methods: the mice were then divided into four groups, one control group and three FEU treated groups. The control group received saline solution, whilst the treated groups received different doses of FEU (5, 15 and 45 mg/kg BW) for 28 days, followed by being forced to undergo swimming test, with measurements taken of various biochemical parameters. Results showed that FEU could increase reduced glutathione (GSH) levels, GSH/oxidized glutathione (GSSG) ratio, superoxide dismutase (SOD) and catalase (CAT) activities, decrease xanthine oxidase (XO) activities, GSSG, malondialdehyde (MDA) and 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels. The finding of the study suggests that FEU possess protective effects against exhaustive exercise-induced oxidative stress and oxidative injury, which might be important in preventing loss of cellular function and warrants quick recovery after sports competition.
Cortex Eucommiae is well known in traditional Chinese herbal medicine. The present study investigated the effects of polysaccharides from Cortex Eucommiae (PCE) on exhaustive exercise-induced oxidative stress in mice by measuring the changes in the activities of superoxide dismutase (SOD), glutathione peroxidase (GPX) and catalase (CAT) and levels of malondialdehyde (MDA) and 8-hydroxydeoxyguanosine (8-OHdG). The mice were randomly divided into four groups: a negative control group, a low-dose PCE intervention group, a medium-dose PCE intervention group and a high-dose PCE intervention group. The mice in the control group were given distilled water whereas those in the three intervention groups were given different doses of PCE (10, 50 and 100 mg/kg). After 28 days, the mice were made to perform an exhaustive swimming exercise. Changes in the activities of the main antioxidant enzymes and the levels of MDA and 8-OHdG in the blood, liver and muscle of the mice were measured. The results of the study showed that PCE increased the activities of SOD, GPX and CAT and decreased the levels of MDA and 8-OHdG in mice, suggesting that PCE has a protective effect on exhaustive exercise- induced oxidative stress.
This study aims to assess the effects of Achyranthes bidentata polysaccharides (ABPS) on the expression of bcl-2 and bax in hepatic tissues after exhaustive exercise in order to provide theoretical support for the application of ABPS in the field of sports nutrition. Thirty male Sprague-Dawley rats were randomized into three groups, each consisting of 10 rats: Normal control group (NCG), Exhausting exercises control group (EECG), ABPS treated group (ATG). ABPS were fed orally by gastric intubation to rats of ABPS treated group (ATG) once daily for 7 days. Control animals (EECG and NCG) received the same amount of isotonic sodium chloride solution. Exhaustive exercise was performed on a rodent treadmill. The SP (streptavidin peroxidase) method for immunohistochemical staining was adopted to test the protein expression of bax and bcl-2 in the hepatic tissues of the rats. Exhausting exercises increased bax protein expression of hepatic tissues of rats and bax/bcl-2 ratio dramatically, but a decreased bcl-2 protein expression. In the rats fed ABPS orally by gastric intubation, the bax protein expression and bax/bcl-2 ratio obviously decreased, while bcl-2 protein expression increased. The result indicated that bax and bcl-2 co-regulated the exercise-induced hepatocyte apoptosis. Feeding ABPS orally by gastric intubation to rats can inhibit the hepatocyte apoptosis in exhaustive exercise.
Biology of sport,2015年32(3):187-92 ISSN：0860-021X
[Qiao, D.; Hu, Y.; Liu, X.] Beijing Normal Univ, Coll Phys Educ & Sports, Beijing 100875, Peoples R China.;[Hu, Y.] Cent S Univ, Dept Phys Educ, Changsha 410083, Hunan, Peoples R China.
[Qiao, D.] Beijing Normal Univ, Coll Phys Educ & Sports, Beijing 100875, Peoples R China.
central fatigue;immunohistochemistry;microdialysis;monoamine neurotransmitters;subthalamic nucleus (STN)
The purpose of the study was to explore the mechanism underlying the enhanced subthalamic nucleus (STN) neural activity during exhausting exercise from the perspective of monoamine neurotransmitters and changes of their corresponding receptors. Rats were randomly divided into microdialysis and immunohistochemistry study groups. For microdialysis study, extracellular fluid of the STN was continuously collected with a microdialysis probe before, during and 90 min after one bout of exhausting exercise. Dopamine (DA) and 5-hydroxytryptamine (5-HT) levels were subsequently detected with high-performance liquid chromatography (HPLC). For immunohistochemistry study, the expression of DRD2 and HT2C receptors in the STN, before, immediately after and 90 min after exhaustion was detected through immunohistochemistry technique. Microdialysis study results showed that the extracellular DA and 5-HT neurotransmitters increased significantly throughout the procedure of exhausting exercise and the recovery period (P<0.05 or P<0.01). Immunohistochemistry study results showed that the expression levels of DRD2 and HT2C in the rat STN immediately after exhausting exercise and at the time point of 90 min after exhaustion were both higher than those of the rest condition, but the difference was not significant (P>0.05). Our results suggest that the increased extracellular DA and 5-HT in the STN might be one important factor leading to the enhanced STN neural activity and the development of fatigue during exhausting exercise. This study may essentially offer useful evidence for better understanding of the mechanism of the central type of exercise-induced fatigue.
The present study was designed to evaluate the anti-fatigue effects of genistein in mice subjected to the forced swimming test. Mice were divided into four groups, i.e., one control group and three genistein treated groups. The treated groups were received doses of genistein (7, 15 and 30 mg/kg) dissolved in 1.0 mL of physiological saline, and the control group received same volume of physiological saline via oral gavage once a day. After 28 days, the mice were subjected to the forced swimming test, along with the determination of exhaustive swimming times and some biochemical parameters. The results showed that genistein could prolong exhaustive swimming time of mice, which was accompanied by decreases in blood lactic acid (BLA) and serum urea nitrogen (SUN) levels, increases in liver and muscle glycogen contents, and superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) levels in serum. This indicated that genistein had anti-fatigue effects.