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Preparative isolation and purification of seven main antioxidants from Eucommia ulmoides Oliv. (Du-zhong) leaves using HSCCC guided by DPPH-HPLC experiment

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WOS被引频次:83
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成果类型:
期刊论文
作者:
Dai, Xingping;Huang, Qiong;Zhou, Boting;Gong, Zhicheng*;Liu, Zhaoqian;Shi, Shuyun
通讯作者:
Gong, Zhicheng
作者机构:
[Dai, Xingping] Cent S Univ, Key Lab Tradit Chinese Med Gan Organ SATCM, Dept Integrated Tradit Chinese & Western Med, Changsha 410008, Hunan, Peoples R China.
[Shi, Shuyun; Liu, Zhaoqian; Gong, Zhicheng; Zhou, Boting; Huang, Qiong] Cent S Univ, Hunan Key Lab Pharmacogenet, Inst Clin Pharmacol, Changsha 410078, Hunan, Peoples R China.
[Gong, Zhicheng] Cent S Univ, Dept Pharm, Xiangya Hosp, Changsha 410008, Hunan, Peoples R China.
[Shi, Shuyun] Cent S Univ, Sch Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.
通讯机构:
[Gong, Zhicheng] Cent S Univ, Hunan Key Lab Pharmacogenet, Inst Clin Pharmacol, Changsha 410078, Hunan, Peoples R China.
语种:
英文
关键词:
Eucommia ulmoides;Antioxidant;DPPH-HPLC;HSCCC;Iridoid;Phenylpropanoid;Flavonoid
期刊:
Food Chemistry
ISSN:
0308-8146
年:
2013
卷:
139
期:
1-4
页码:
563-570
文献类别:
WOS:Article;EI:Journal article (JA)
所属学科:
ESI学科类别:农业科学;WOS学科类别:Chemistry, Applied;Food Science & Technology;Nutrition & Dietetics
入藏号:
WOS:000318200900078;EI:20131516202666
基金类别:
National Scientific Foundation of China [21275163, 21005089]; Science and Technology Program of Hunan Province, China [2012FJ2006]; freedom explore Program of Central South University [201012200015]; Shenghua Yuying Project of Central South University
机构署名:
本校为第一且通讯机构
院系归属:
化学化工学院
湘雅药学院
湘雅医院(第一临床学院)
摘要:
Seven antioxidants were purified from Eucommia ulmoides Oliv. leaves using HSCCC guided by DPPH-HPLC experiment. HSCCC was successfully used to separate target antioxidants by three runs with different solvent systems after D101 column chromatography fractionation. Ethyl acetate-n-butanol-water (1:2:3, v/v/v) was selected as the optimum solvent system to purify geniposidic acid. Ethyl acetate-ethanol-water (4:1:5, v/v/v) was used to isolate caffeic acid, chlorogenic acid and ferulic acid. While three flavonoids, quercetin-3-O- sambubioside, rutin and isoquercitrin were purified by petroleum ether-ethyl acetate-methanol-water (1:5:1:5, v/v/v/v). The structures were identified by MS and NMR. Antioxidant activities were assessed, and compounds 2-7 showed strong antioxidant activities. This is the first report about separation of antioxidants from E. ulmoides leaves by HSCCC. The results indicated that the combinative methods using DPPH-HPLC and HSCCC could be widely applied for screening and isolation of antioxidants from complex extracts. ©2013 Elsevier Ltd. All rights reserved.
参考文献:
Ammar R. B., 2009, FOOD CHEM, V116, P164
Arapitsas P, 2008, FOOD CHEM, V110, P1041, DOI 10.1016/j.foodchem.2008.03.014
Cho EJ, 2003, PHYTOMEDICINE, V10, P544, DOI 10.1078/094471103322331520
Conforti F, 2011, FOOD CHEM, V129, P1413, DOI 10.1016/j.foodchem.2011.05.085
Costa F. das N., 2010, Journal of Separation Science, V33, P336, DOI 10.1002/jssc.200900632

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